{"product_id":"cst-14396s","title":"CST,  14396S, M-RIP (D8G8R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MPRIP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, M-RIP (D8G8R) Rabbit Monoclonal Antibody (CST #14396) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nM-RIP (D8G8R) Rabbit Monoclonal Antibody recognizes endogenous levels of total M-RIP protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro62 of human M-RIP protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMyosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics (1-3). M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS\/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA (1-3). Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility (4,5). M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho\/ROCK, and actin (2,6). Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nKIAA0864; M-RIP; MPRIP; MRIP; myosin phosphatase Rho interacting protein; Myosin phosphatase Rho-interacting protein; myosin phosphatase-Rho interacting protein; p116Rip; Rho interacting protein 3; Rho-interacting protein 3; RHOIP3; RIP3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 130-140\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797609042089,"sku":"14396S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14396s","provider":"Iright","version":"1.0","type":"link"}