CST, 14412T, Ras (G12V Mutant Specific) (D2H12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KRas (Gly12Val) mutation. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Ras (G12V Mutant Specific) (D2H12) Rabbit Monoclonal Antibody (CST #14412) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ras (G12V Mutant Specific) (D2H12) Rabbit Monoclonal Antibody recognizes endogenous levels of Ras G12V mutant protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to G12V mutant sequence of human Ras protein. Background The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAPs) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6). Additional oncogenic mutations of Ras have been observed at varying frequencies at codons 12, 13, and 61. The Gly12 to Val12 (G12V) mutation has been detected in a number of different cancers, including colorectal and thyroid cancer (7,8). Alternate Names 'C-K-RAS; c-K-ras; c-Ki-ras; c-Ki-ras2; c-Kirsten-ras protein; cellular c-Ki-ras2 proto-oncogene; cellular transforming proto-oncogene; CFC2; GTPase KRas; GTPase KRas, N-terminally processed; K-Ras; K-Ras 2; K-ras p21 protein; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; Ki-Ras; Kirsten rat sarcoma viral oncogene homolog; Kirsten rat sarcoma viral proto-oncogene; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; KRAS; KRAS proto-oncogene, GTPase; KRAS1; KRAS2; NS; NS3; OES; oncogene KRAS2; PR310 c-K-ras oncogene; RALD; RASK; RASK2; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG