CST, 14466S, RAP80 (D1T6Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RAP80. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, RAP80 (D1T6Q) Rabbit Monoclonal Antibody (CST #14466) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity RAP80 (D1T6Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total RAP80 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly321 of human RAP80 protein. Background The breast cancer type 1 susceptibility protein (BRCA1) is an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of the DNA damage response and DNA repair. BRCA1 protein forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for regulation of BRCA1 protein function. The BRCA1-RAP80 complex (BRCA1 A complex) includes RAP80, BRCC36, BRE, Abraxas, and NBA1 and functions in G2/M phase checkpoint control (reviewed in 1,2). The ubiquitously expressed receptor-associated protein 80 (RAP80, UIMC1) is required for recruitment and stability of the BRCA1 A complex at sites of DNA damage (3). Research studies indicate that the absence of RAP80 in cells results in increased sensitivity to the topoisomerase II inhibitor etoposide (4). In the absence of functional RAP80, BRCA1 A complex function is suppressed and cells become more sensitive to DNA damage-induced genome instability (5,6). Phosphorylation of RAP80 by CDK1/Cyclin B at Ser177 regulates RAP80 function at the mitotic checkpoint (7). A naturally occurring in-frame deletion mutant within RAP80 likely alters RAP80 protein-protein interactions and is associated with an increase in chromosomal abnormalities (8,9). Alternate Names BRCA1-A complex subunit RAP80; RAP80; receptor associated protein 80; Receptor-associated protein 80; retinoid x receptor interacting protein; Retinoid X receptor-interacting protein 110; RXRIP110; ubiquitin interaction motif containing 1; Ubiquitin interaction motif-containing protein 1; UIMC1; X2HRIP110 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG