CST, 14468T, ELL (D7N6U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ELL. Validated for WB,IP,IF,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, ELL (D7N6U) Rabbit Monoclonal Antibody (CST #14468) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:1600 Chromatin IP: 1:100 Chromatin IP-seq: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq Specificity / Sensitivity ELL (D7N6U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ELL protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp399 of human ELL protein. Background The super elongation complex (SEC) plays a critical role in regulating RNA polymerase II (RNAPII) transcription elongation (1). The SEC is composed of AFF4, AFF1/AF4, MLLT3/AF9, and MLLT1/ENL proteins. The pathogenesis of mixed lineage leukemia is often associated with translocations of the SEC subunits joined to the histone H3 Lys4 methyltransferase mixed lineage leukemia ( ) gene (1-4). The SEC has been found to contain RNAPII elongation factors eleven-nineteen lysine-rich leukemia (ELL), ELL2, and ELL3, along with the associated factors EAF1 and EAF2, which can increase the catalytic rate of RNAPII transcription , (1,2,5-7). The SEC positive transcription elongation factor b (P-TEFb) phosphorylates the carboxy-terminal domain within the largest subunit of RNAP II at Ser2 of the heptapeptide repeat. The SEC negative transcription elongation factors, DRB-induced stimulating factor (DSIF) and negative elongation factor (NELF), signal the transition from transcription initiation and pausing to productive transcription elongation (2,8-10). The chromosomal translocation of with the members of the SEC leads to SEC recruitment to MLL regulated genes, such as the highly developmentally regulated genes, implicating the misregulation and overexpression of these genes as underlying contributors to leukemogenesis (1,2,9,11). Alternate Names C19orf17; DKFZp434I1916; Eleven-nineteen lysine-rich leukemia protein; ELL; ELL gene (11-19 lysine-rich leukemia gene); ELL/KMT2A fusion protein; ELL1; elongation factor for RNA polymerase II; elongation factor RNA polymerase II; KMT2A/ELL fusion protein; MEN; PPP1R68; protein phosphatase 1, regulatory subunit 68; RNA polymerase II elongation factor ELL Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG