CST, 14488T, ATP6V1B2 (D3O7Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATP6V1B2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ATP6V1B2 (D3O7Q) Rabbit Monoclonal Antibody (CST #14488) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ATP6V1B2 (D3O7Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATP6V1B2 protein. This antibody does not cross-react with ATP6V1B1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ATP6V1B2 protein. Background Eukaryotic cells contain ATP-driven proton pumps known as vacuolar H+-ATPases (V-ATPases) that acidify intracellular compartments and translocate protons across the plasma membrane (1,2). Intracellular v-ATPases play an important role in endocytosis and intracellular membrane trafficking, while plasma membrane v-ATPases are important in processes such as urinary acidification and bone resorption (1,2). Vacuolar ATPase enzymes are large, heteromultimeric protein complexes with component proteins found in either the V1 peripheral domain or the V0 integral domain (2). The cytoplasmic V1 domain contains a hexamer of A and B catalytic subunits, as well as a number of other protein subunits required for ATPase assembly and ATP hydrolysis. The integral V0 v-ATPase domain exhibits protein translocase activity and is responsible for transport of protons across the membrane (2). Research studies show that the v-ATPases ATP6V0c, ATP6V0d1, ATP6V1A, ATP6V1B2, and ATP6V1D interact with the Ragulator protein complex and are essential for amino acid induced activation of mTORC1 on the surface of lysosomes (3). Two isoforms of the B subunit are found in humans, ATP6V1B1 and ATP6V1B2. The ATP6V1B1 protein is expressed primarily in the kidney, with mutations in the corresponding gene responsible for a form of renal tubular acidosis associated with progressive hearing loss (4,5). ATP6V1B2 protein exhibits a broader range of expression, localized to kidney, brain, pancreas, and other tissues (4). Alternate Names ATP6B1B2; ATP6B2; ATP6V1B2; ATPase H+ transporting V1 subunit B2; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2; DOOD; Endomembrane proton pump 58 kDa subunit; H+ transporting two-sector ATPase; HO57; testicular secretory protein Li 65; V-ATPase B2 subunit; V-ATPase subunit B 2; V-type proton ATPase subunit B, brain isoform; vacuolar H+-ATPase 56,000 subunit; Vacuolar proton pump subunit B 2; VATB; VATB2; Vma2; VPP3; ZLS2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG