CST, 14495T, EDC3 (D2T7U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EDC3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, EDC3 (D2T7U) Rabbit Monoclonal Antibody (CST #14495) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity EDC3 (D2T7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total EDC3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly322 of human EDC3 protein. Background Enhancer of mRNA decapping 3 (EDC3) was originally identified from as a protein essential to mRNA decapping prior to 5'-3' mRNA degradation (1). In human cells, EDC3 is found within cytoplasmic processing (P) bodies as part of complexes that include DCP1, DCP2, EDC4/Ge-1, and DDX6/RCK (2). EDC3 and DCP2 interact with TTP, an activator of AU-rich-element (ARE)-mediated decay pathway, to promote decapping and degradation of ARE mRNA (2). In addition, research studies indicate that EDC3 may play a role in the premature termination of RNA polymerase II transcription (3). Alternate Names EDC3; enhancer of mRNA decapping 3; enhancer of mRNA decapping 3 homolog; enhancer of mRNA decapping 3 homolog (S. cerevisiae); Enhancer of mRNA-decapping protein 3; FLJ21128; FLJ31777; hYjeF_N2; hYjeF_N2-15q23; LSM16; LSM16 homolog; LSM16 homolog (EDC3, S. cerevisiae); MRT50; YJDC; yjeF domain containing; YjeF domain-containing protein 1; YjeF N-terminal domain-containing protein 2; YjeF_N2; YJEFN2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG