CST, 14498T, OAS1 (D1W3A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying OAS1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, OAS1 (D1W3A) Rabbit Monoclonal Antibody (CST #14498) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity OAS1 (D1W3A) Rabbit Monoclonal Antibody recognizes endogenous levels of total OAS1 protein. This antibody cross-reacts with an unidentified protein of 100 kDa in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp90 of human OAS1 protien. Background 2'-5'-oligoadenylate synthetase 1 (OAS1) is an antiviral protein induced by type 1 interferon that plays a key role in the cellular innate immune response (1). The OAS family of proteins includes OAS1, OAS2, OAS3, and OASL in humans (2). The OAS1 enzyme produces the second messenger 2'-5'-linked oligoadenylate in response to cytosolic dsRNA. These 2'-5'-linked oligoadenylates bind to the ribonuclease RNase L, which then degrades viral and cellular RNA (3). Research studies indicate that the OAS1 system inhibits protein synthesis and induces apoptosis in virally infected cells, which limits viral infection (4). Alternative splicing generates multiple isoforms of human OAS1, including p41 and the canonical p46 (5,6). Polymorphisms in the corresponding OAS1 gene have been examined for possible association with increased susceptibility to type 1 diabetes mellitus, multiple sclerosis, and infection by viral pathogens (7,8). Alternate Names (2-5')oligo(A) synthase 1; (2-5')oligo(A) synthetase 1; 2-5A synthase 1; 2-5A synthetase 1; 2'-5' oligoadenylate synthetase 1 p48 isoform; 2'-5' oligoadenylate synthetase 1 p52 isoform; 2'-5'-oligoadenylate synthase 1; 2'-5'-oligoadenylate synthetase 1; 2'-5'-oligoadenylate synthetase 1, 40/46kDa; 2'-5'-oligoisoadenylate synthetase 1; 2',5'-oligo A synthetase 1; 2',5'-oligoadenylate synthetase 1, 40/46kDa; E16 (2'-5') oligo A synthetase; E18/E16; IFI-4; OAS1; OIAS; OIASI; p46/p42 OAS Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40, 44 Source/Isotype: Rabbit IgG