CST, 14501S, EAAT3 (E1E6M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EAAT3. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, EAAT3 (E1E6M) Rabbit Monoclonal Antibody (CST #14501) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:800 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 30102 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity EAAT3 (E1E6M) Rabbit Monoclonal Antibody recognizes endogenous levels of total EAAT3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val510 of human EAAT3 protein. Background During neurotransmission, glutamate is released from vesicles of the presynaptic cell, and glutamate receptors (e.g., NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing postsynaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels (1,2). In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion (1,2). Five EAATs (EAAT1-5) have been identified. EAAT1 and EAAT2 are expressed mainly in glia, while EAAT3, EAAT4, and EAAT5 are considered to be neuronal transporters (2). EAAT3 is found in the perisynaptic areas and cell bodies of glutamatergic and GABAergic neurons (3). Research studies have implicated abnormal EAAT3 expression in the pathophysiology of Schizophrenia (4,5). Alternate Names DCBXA; EAA3; EAAC1; EAAT3; excitatory amino acid carrier 1; Excitatory amino acid transporter 3; excitatory amino acid transporter-3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; SCZD18; SLC1A1; Sodium-dependent glutamate/aspartate transporter 3; solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1; Solute carrier family 1 member 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG