CST, 14558S, Synaptotagmin-1 (D33B7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SYT1. Validated for WB,WB,IP,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, Synaptotagmin-1 (D33B7) Rabbit Monoclonal Antibody (CST #14558) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Synaptotagmin-1 (D33B7) Rabbit Monoclonal Antibody recognizes endogenous levels of total synaptotagmin-1 protein. This antibody may also cross-react with an unidentified protein of approximately 45 kDa. This antibody is not recommended for immunohistochemical analysis of human tissues. Non-specific staining was observed in mouse pancreatic islets by immunohistochemistry. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg400 of human synaptotagmin-1 protein. Background Synaptotagmin-1 (SYT1) is an integral membrane protein found in synaptic vesicles thought to play a role in vesicle trafficking and exocytosis (1). Individual SYT1 proteins are composed of an amino-terminal transmembrane region, a central linker region and a pair of carboxy-terminal C2 domains responsible for binding Ca (2). The C2 domains appear to be functionally distinct, with the C2A domain responsible for regulating synaptic vesicle fusion in a calcium-dependent manner during exocytosis while the C2B domain allows for interaction between adjacent SYT1 proteins (3). Because synaptotagmin 1 binds calcium and is found in synaptic vesicles, this integral membrane protein is thought to act as a calcium sensor in fast synaptic vesicle exocytosis. Evidence suggests possible roles in vesicle-mediated endocytosis and glucose-induced insulin secretion as well (4,5). SYT1 binds several different SNARE proteins during calcium-mediated vesicle endocytosis and an association between SYT1 and the SNARE protein SNAP-25 is thought to be a key element in vesicle-mediated exocytosis (6). Alternate Names BAGOS; DKFZp781D2042; p65; SVP65; synaptotagmin 1; Synaptotagmin I; Synaptotagmin-1; SYT; SYT1; SytI Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG