CST, 14623T, Neurofibromin 1 (D7R7D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NF1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Neurofibromin 1 (D7R7D) Rabbit Monoclonal Antibody (CST #14623) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Neurofibromin 1 (D7R7D) Rabbit Monoclonal Antibody recognizes endogenous levels of total neurofibromin 1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro360 of human neurofibromin 1 protein. Background Neurofibromin is a Ras-specific GTPase activating protein (RasGAP), down-regulating Ras signaling (1). Studies have shown, that mutations in inhibit its activity, resulting in benign tumors such as neurofibromas, which may form along nerves throughout the body resulting in neurofibromatosis type 1 (NF1) (2). NF1 is one of the most common autosomal dominant diseases however it remains unclear how mutation of may lead to other features of NF1 (3). In addition, mutations occur in 5-10% of human sporadic malignancies such as glioblastomas, lung adenocarcinomas, melanomas, breast and ovarian cancers, and acute myeloid leukemias. Mutations in can cause resistance to therapies including chemotherapy and radiation therapy (3). Alternate Names DKFZp686J1293; FLJ21220; neurofibromatosis 1; neurofibromatosis-related protein NF-1; Neurofibromin; neurofibromin 1; neurofibromin truncated; NF1; NFNS; truncated neurofibromin 1; VRNF; WSS Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 320 Source/Isotype: Rabbit IgG