CST, 14688S, FRMD6 (D8X3R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FRMD6. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FRMD6 (D8X3R) Rabbit Monoclonal Antibody (CST #14688) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FRMD6 (D8X3R) Rabbit Monoclonal Antibody recognizes endogenous levels of total FRMD6 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe608 of human FRMD6 protein. Background FERM domain-containing protein 6 (FRMD6, Willin) belongs to the 4.1 superfamily, a group of proteins characterized by the presence of a Band 4.1, Ezrin, Radixin, Moesin (FERM) domain in the amino-terminal region (1). Members of this protein superfamily, which also includes Merlin, Ezrin, Radixin, Moesin and Talin, function primarily as adaptor proteins that link plasma membrane-associated proteins to the actin cytoskeleton (2). FRMD6 is considered orthologous to the Expanded (Ex) protein, which is a 4.1 family protein that functions together with Merlin and Kibra to regulate Hippo signaling, upstream of the Salvador/Warts/Hippo core kinase cassette (3). Research studies suggest a similar role for FRMD6 in regulating the Hippo pathway in mammals. For example, ectopic expression of FRMD6 leads to increased phosphorylation of MST1/2, LATS1, and YAP, which is indicative of Hippo pathway activation. Loss of FRMD6 expression in MCF10A cells induces a phenotype change resembling epithelial-mesenchymal transition (4). Alternate Names 4.1 ezrin radixin moesin (FERM)-containing protein; c14_5320; C14orf31; EX1; expanded homolog; FERM domain containing 6; FERM domain-containing protein 6; FRMD6; MGC17921; Willin Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG