{"product_id":"cst-14716t","title":"CST,  14716T, Phospho-Sin1 (Thr86) (D4U9L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SIN1 (Thr86) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Sin1 (Thr86) (D4U9L) Rabbit Monoclonal Antibody (CST #14716) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Sin1 (Thr86) (D4U9L) Rabbit Monoclonal Antibody recognizes endogenous levels of Sin1 protein only when phosphorylated at Thr86.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding phosphorylated Thr86 of human Sin1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase that is a component of two different complexes. The mTOR complex 1 (mTORC1), a target of rapamycin, contains mTOR, GβL, and raptor. mTORC2, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTORC2 complex phosphorylates Ser473 of Akt\/PKB (2). This phosphorylation is essential for full Akt\/PKB activation. Furthermore, rictor siRNA knockdown inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). mTORC2 has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). In addition, phosphorylation of Sin1 at Thr86 by Akt\/PKB was shown to regulate the activity of mTORC2 in adipocytes upon stimulation by growth factors (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nJC310; MAPK associated protein 1; MAPKAP1; MEKK2-interacting protein 1; MGC2745; MIP1; Mitogen-activated protein kinase 2-associated protein 1; mitogen-activated protein kinase associated protein 1; mSIN1; ras inhibitor MGC2745; SAPK-interacting protein 1; SIN1; SIN1b; SIN1g; stress-activated map kinase interacting protein 1; Stress-activated map kinase-interacting protein 1; stress-activated protein kinase-interacting 1; Target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 78, 74\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797615268009,"sku":"14716T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14716t","provider":"Iright","version":"1.0","type":"link"}