CST, 14719T, CYP1A2 (D2V7S) Mouse Monoclonal Antibody

Monoclonal Antibody for studying CYP1A2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CYP1A2 (D2V7S) Mouse Monoclonal Antibody (CST #14719) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CYP1A2 (D2V7S) Mouse Monoclonal Antibody recognizes endogenous levels of total CYP1A2 protein. This antibody does not cross-react with CYP1A1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with 3-methylcholanthrene-induced rat liver microsomal fractions. Background Cytochrome P450 (CYP) is a family of enzymes that contain a heme group (1). These enzymes, when reduced and bound by carbon monoxide, maximally absorb light of 450 nm (1). Type I cytochrome P450s are found in mitochondria and function in the biosynthesis of essential molecules (1). Type II cytochrome P450s are found in endoplasmic reticulum (1). Some type II cytochrome P450s play a role in the biosynthesis of essential molecules whereas others metabolize xenobiotics (1). Research studies show that cytochrome P450s form various heteromeric complexes with other members of the P450 family influencing their catalytic activities (2-4). CYP1A2 is in the endoplasmic reticulum of hepatocytes and responsible for the breakdown of a variety of xenobiotic substances and bioactivation of carcinogens (2, 5). CYP1 enzymes, including CYP1A2, have been implicated in smoking-related osteoporosis (6). A meta-analysis shows that a particular polymorphism in is potentially linked to increased cancer risk (5). Alternate Names aryl hydrocarbon hydroxylase; Cholesterol 25-hydroxylase; CP12; CP1A2; CYP1A2; CYPIA2; Cytochrome P(3)450; Cytochrome P450 1A2; Cytochrome P450 4; cytochrome P450 family 1 subfamily A member 2; Cytochrome P450-P3; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; Hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P3-450; P450 form 4; P450(PA); xenobiotic monooxygenase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Mouse IgG1