CST, 14783T, VANGL1 (D1J7X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VANGL1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, VANGL1 (D1J7X) Rabbit Monoclonal Antibody (CST #14783) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VANGL1 (D1J7X) Rabbit Monoclonal Antibody recognizes endogenous levels of total VANGL1 protein. This antibody does not cross-react with VANGL2 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu309 of human VANGL1 protein. Background Van Gogh-like proteins (VANGL1, VANGL2) are human orthologs of Van Gogh (Vang/Stbm), a multi-pass transmembrane protein that is required to establish cell polarity in embryonic eyes, legs, and bristles (1,2). As in , mammalian VANGL proteins are core components of the planar cell polarity (PCP) pathway that promotes asymmetric orientation of cells across a planar surface, and drives convergence-extension movements that are critical for tissue morphogenesis (3). Mutations in the human gene have been identified in patients diagnosed with neural tube defects (e.g., spina bifida), providing evidence that VANGL1 plays a role in human embryonic morphogenesis (4,5). These findings are supported by genetic studies in mice, where mutations in both and result in neural tube defects (6,7). A possible role for VANGL in tumor progression is suggested by an increased expression of mRNA in breast cancer patients with an elevated risk of relapse (8). Alternate Names KAI1 C-terminal interacting tetraspanin; KITENIN; Loop-tail protein 2 homolog; LPP2; MGC5338; STB2; STBM2; Strabismus 2; Van Gogh-like protein 1; vang-like 1 (van gogh, Drosophila); Vang-like protein 1; VANG1; VANGL planar cell polarity protein 1; VANGL1 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG