{"product_id":"cst-14791s","title":"CST,  14791S, Phospho-Zap-70 (Tyr319)\/Syk (Tyr352) (65E4) Rabbit Monoclonal Antibody (PE Conjugate)","description":"Monoclonal Antibody for studying ZAP70 (Tyr319) phosphate\/Syk (Tyr352) phosphate. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Phospho-Zap-70 (Tyr319)\/Syk (Tyr352) (65E4) Rabbit Monoclonal Antibody (PE Conjugate) (CST #14791) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Zap-70 (Tyr319)\/Syk (Tyr352) (65E4) Rabbit Monoclonal Antibody (PE Conjugate) detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. This antibody conjugate cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; DKFZp313N1010; FLJ17670; FLJ17679; FLJ25043; FLJ37489; IMD48; KSYK; p72-Syk; spleen associated tyrosine kinase; Spleen tyrosine kinase; SRK; STCD; STD; SYK; Syk-related tyrosine kinase; Tyrosine-protein kinase SYK; Tyrosine-protein kinase ZAP-70; TZK; ZAP-70; ZAP70; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800345956521,"sku":"14791S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14791s","provider":"Iright","version":"1.0","type":"link"}