{"product_id":"cst-14795t","title":"CST,  14795T, DTX3L (D5F2J) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying DTX3L. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DTX3L (D5F2J) Rabbit Monoclonal Antibody (CST #14795) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDTX3L (D5F2J) Rabbit Monoclonal Antibody recognizes endogenous levels of total DTX3L protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val22 of human DTX3L protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe E3 ubiquitin-protein ligase DTX3L (BBAP) is a deltex (DTX) family ligase that binds B aggressive lymphoma 1 (BAL1) to mediate the monoubiquitination of histone H4 Lys91 in response to DNA damage (1,2). The early recruitment of poly-ADP-ribose polymerase 1 (PARP1) protein to sites of DNA damage leads to poly-ADP-ribosylation (PARylation) of multiple target proteins. This results in the subsequent recruitment of DTX3L\/BAL1 through binding of the BAL macrodomain to PARylated proteins. DTX3L\/BAL1 then mediates the monoubiquitination of histone H4 Lys91 that is required for subsequent methylation of histone H4 Lys20 and recruitment of p53-binding protein 1 (1-3). DTX3L and BAL1 are highly expressed in malignant B-cell lymphomas and act to confer chemotherapy-resistance by protecting cells from DNA damaging agents (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB-lymphoma- and BAL-associated protein; BBAP; deltex 3 like, E3 ubiquitin ligase; deltex 3-like; deltex 3-like (Drosophila); deltex E3 ubiquitin ligase 3L; DTX3L; E3 ubiquitin-protein ligase DTX3L; Protein deltex-3-like; rhysin 2; Rhysin-2; Rhysin2; RING-type E3 ubiquitin transferase DTX3L; RNF143\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 84\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797617004713,"sku":"14795T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14795t","provider":"Iright","version":"1.0","type":"link"}