CST, 14805T, CIP2A (D1M3H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CIP2A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CIP2A (D1M3H) Rabbit Monoclonal Antibody (CST #14805) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CIP2A (D1M3H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CIP2A protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val342 of human CIP2A protein. Background Protein phosphatase 2A (PP2A) is a trimeric protein phosphatase and tumor suppressor that regulates the phosphorylation status of a wide variety of phosphoproteins. PP2A targets include many that play a role in the maintenance and progression of cancer (1). The cancerous inhibitor of protein phosphatase 2A (CIP2A) is a single pass membrane protein that binds the PP2A catalytic subunit to inhibit PP2A phosphatase activity (2). CIP2A is normally expressed at low levels in normal cells and tissues, but is elevated in human malignancies where it is thought to be oncogenic. Research studies demonstrate aberrant CIP2A expression in multiple tumor types, including those derived from the head and neck, liver, colon, lung, osteosarcoma, pancreatic, breast, and myeloid cancers (reviewed in 3). This evidence suggests that CIP2A interacts with many proteins that may play a role in cancer maintenance and progression (3). Additional studies indicate that CIP2A inhibits PP2A-mediated dephosphorylation of the proto-oncogene Myc at Ser64, which stabilizes and prevents proteolytic degradation of the Myc transcription factor (4). Alternate Names Cancerous inhibitor of PP2A; cancerous inhibitor of protein phosphatase 2A; cell proliferation regulating inhibitor of protein phosphatase 2A; cellular inhibitor of PP2A; CIP2A; FLJ12850; KIAA1524; MGC163436; p90; p90 autoantigen; Protein CIP2A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG