{"product_id":"cst-14807s","title":"CST,  14807S, APC10 Antibody","description":"Polyclonal Antibody for studying APC10. Validated for Western Blotting. Highly specific and rigorously validated in-house, APC10 Antibody (CST #14807) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAPC10 Antibody recognizes endogenous levels of total APC10 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human APC10 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nEukaryotic cell proliferation depends strictly upon the E3 ubiquitin ligase activity of the anaphase promoting complex\/cyclosome (APC\/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. The APC\/C complex promotes the assembly of polyubiquitin chains on substrate proteins in order to target these proteins for degradation by the 26S proteasome (1,2). The vertebrate APC\/C complex consists of as many as 15 subunits, including multiple scaffold proteins, two catalytic subunits (APC2, APC11), and a number of proteins responsible for substrate recognition (3). All E3 enzymes, including APC\/C, utilize ubiquitin residues activated by E1 enzymes and transferred to E2 enzymes. Research studies indicate that APC\/C interacts with the E2 enzymes UBE2S and UBE2C via the RING-finger domain-containing subunit APC11 (4-6). APC\/C function relies on multiple cofactors, including an APC\/C coactivator formed by the cell division control protein 20 homolog (CDC20) and Cdh1\/FZR1. The CDC20\/Cdh1 coactivator is responsible for recognition of APC\/C substrates through interaction with specific D-box and KEN-box recognition elements within these substrates (7-9). Anaphase-promoting complex subunit 10 (APC10, DOC1) is a highly conserved, core component of the anaphase promoting complex\/cyclosome (10,11). Research studies indicate that APC10 participates in substrate recognition by the APC\/C (3,12,13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nANAPC10; anaphase promoting complex subunit 10; Anaphase-promoting complex subunit 10; APC10; Cyclosome subunit 10; DKFZp564L0562; DOC1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 24\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797617234089,"sku":"14807S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14807s","provider":"Iright","version":"1.0","type":"link"}