CST, 14817S, OTUD7B/Cezanne-1 Antibody

Polyclonal Antibody for studying Cezanne. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, OTUD7B/Cezanne-1 Antibody (CST #14817) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity OTUD7B/Cezanne-1 Antibody recognizes endogenous levels of total OTUD7B/Cezanne-1 protein. This antibody does not cross-react with either OTUD7A/Cezanne-2 or OTUD7C/A20 proteins. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human OTUD7B/Cezanne-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes respectively (1,2). Deubiquitinating enzymes (DUBS) are categorized into five subfamilies based on catalytic domain structure: USP, UCH, OTU, MJD, and JAMM. The deubiquitinase cellular zinc-finger anti-NF-κB (Cezanne-1, OTUD7B) is an OTU family deubiquitinase that contains amino-terminal catalytic and ubiquitin-associated (UBA) domains, and a carboxy-terminal A20-like zinc finger (A20-ZnF) that is involved in ubiquitin binding (3,4). Research studies demonstrate that Cezanne-1 negatively regulates canonical NF-κB signaling induced by TNF receptor signaling by removing K63-linked ubiquitin chains from the RIP1 adaptor protein (5,6). Cezanne-1 negatively regulates non-canonical NF-κB signaling through the deubiquitination and stabilization of the TRAF3 signal transduction protein (7). Additional research suggests that Cezanne-1 is a breast cancer oncogene as the corresponding gene is amplified in a subset of breast cancers and enhances EGFR signaling through a mechanism involving receptor stabilization (8). Alternate Names Cellular zinc finger anti-NF-kappa-B protein; cellular zinc finger anti-NF-kappaB Cezanne; CEZANNE; OTU deubiquitinase 7B; OTU domain containing 7B; OTU domain-containing protein 7B; OTU7B; OTUD7B; ZA20D1; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne; zinc finger, A20 domain containing 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 98 SOURCE: Rabbit