{"product_id":"cst-14817s","title":"CST,  14817S, OTUD7B\/Cezanne-1 Antibody","description":"Polyclonal Antibody for studying Cezanne. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, OTUD7B\/Cezanne-1 Antibody (CST #14817) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nOTUD7B\/Cezanne-1 Antibody recognizes endogenous levels of total OTUD7B\/Cezanne-1 protein. This antibody does not cross-react with either OTUD7A\/Cezanne-2 or OTUD7C\/A20 proteins.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human OTUD7B\/Cezanne-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nProtein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes respectively (1,2). Deubiquitinating enzymes (DUBS) are categorized into five subfamilies based on catalytic domain structure: USP, UCH, OTU, MJD, and JAMM. The deubiquitinase cellular zinc-finger anti-NF-κB (Cezanne-1, OTUD7B) is an OTU family deubiquitinase that contains amino-terminal catalytic and ubiquitin-associated (UBA) domains, and a carboxy-terminal A20-like zinc finger (A20-ZnF) that is involved in ubiquitin binding (3,4). Research studies demonstrate that Cezanne-1 negatively regulates canonical NF-κB signaling induced by TNF receptor signaling by removing K63-linked ubiquitin chains from the RIP1 adaptor protein (5,6). Cezanne-1 negatively regulates non-canonical NF-κB signaling through the deubiquitination and stabilization of the TRAF3 signal transduction protein (7). Additional research suggests that Cezanne-1 is a breast cancer oncogene as the corresponding gene is amplified in a subset of breast cancers and enhances EGFR signaling through a mechanism involving receptor stabilization (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCellular zinc finger anti-NF-kappa-B protein; cellular zinc finger anti-NF-kappaB Cezanne; CEZANNE; OTU deubiquitinase 7B; OTU domain containing 7B; OTU domain-containing protein 7B; OTU7B; OTUD7B; ZA20D1; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne; zinc finger, A20 domain containing 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 98\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797617496233,"sku":"14817S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14817s","provider":"Iright","version":"1.0","type":"link"}