CST, 14950S, MED26 (D4B1X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MED26. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, MED26 (D4B1X) Rabbit Monoclonal Antibody (CST #14950) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity MED26 (D4B1X) Rabbit Monoclonal Antibody recognizes endogenous levels of total MED26 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro120 of human MED26 protein. Background The mediator complex consists of about 25-30 proteins and is thought to facilitate transcription activation by acting as a molecular bridge between the RNA polymerase II (RNAPII) machinery and transcription factors (1). Mediator is recruited to target genes by transcription factors and plays an essential role in the recruitment and stabilization of the RNAPII transcription complex at promoters, as well as the activation of transcription post RNAPII recruitment (1-5). The mediator complex also plays an important role in creating ‘chromatin loops' that occur as a result of interactions between the transcription factor bound at distal enhancers and RNAPII bound at the proximal promoter and works to sustain proper chromatin architecture during active transcription (6-8). Mediator complex subunit 26 (MED26) plays a critical role in the transition of RNAPII from the initiation to elongation phase of transcription. The amino-terminal domain of MED26 is required for recruitment of the ELL- and pTEFb-containing complexes to target genes, which facilitates transcription elongation (9). Alternate Names Activator-recruited cofactor 70 kDa component; ARC70; Cofactor required for Sp1 transcriptional activation subunit 7; cofactor required for Sp1 transcriptional activation, subunit 7 (70kD); cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa; CRSP complex subunit 7; CRSP7; CRSP70; MED26; Mediator complex subunit 26; Mediator of RNA polymerase II transcription subunit 26; Transcriptional coactivator CRSP70 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG