CST, 14954T, Spry2 (D3G1A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Spry2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Spry2 (D3G1A) Rabbit Monoclonal Antibody (CST #14954) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Spry2 (D3G1A) Rabbit Monoclonal Antibody recognizes endogenous levels of total Spry2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro71 of human Spry2 protein. Background The Sprouty (Spry) family of proteins are antagonists of receptor tyrosine kinase (RTK)-induced signaling (1, 2). The Spry proteins play crucial roles in regulating growth and development of living organisms. Since originally discovered in , four human orthologs of Spry proteins (Spry1-4) have been identified. All human Spry proteins possess a conserved carboxyl-terminal cysteine-rich SPR domain, which harbors a signal for protein translocation from cytosol to membrane ruffles (3,4). The SPR domain also enables the Spry proteins to form homo- or hetero-dimers and to interact with other proteins including kinases and phosphatases. The SPR domain is essential for the inhibitory modulation of Spry proteins on RTK signaling (1,2). Studies have shown that several cancers have reduced levels of Spry2 expression implicating Spry2 as a tumor suppressor (5-8). The regulation of Spry2 expression and activity appears to be a complex process involving casein kinase 1, Shp2 phosphatase, and Spry2-interacting partners (9-11). Phosphorylation of Tyr55 residue of Spry2 is required for the inhibitory function of Spry2 in FGF/MAPK signaling (12,13). Alternate Names hSPRY2; IGAN3; MGC23039; Protein sprouty homolog 2; sprouty 2; sprouty homolog 2 (Drosophila); sprouty RTK signaling antagonist 2; Spry-2; SPRY2; SPY2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG