CST, 14957T, HCN2 (D1C6I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HCN2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HCN2 (D1C6I) Rabbit Monoclonal Antibody (CST #14957) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HCN2 (D1C6I) Rabbit Monoclonal Antibody recognizes endogenous levels of total HCN2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala751 of human HCN2 protein. Background The voltage-activated ion channel superfamily includes both cyclic nucleotide-gated (CNG) channels and hyperpolarization-activated cyclic nucleotide-modulated (HCN) channels (1,2). The direct binding of the cyclic nucleotides cAMP and cGMP promotes the gating of these channels, from the closed to an open state. Research studies demonstrate that HCN channels are involved in repetitive firing, resting membrane potential, and dendritic integration in neurons (1,3). HCN proteins are found in homotetramers and heterotetramers composed of HCN1 and HCN2 subunits (4). Tetramerization of HCN proteins results from cAMP binding to the channel, which triggers the release of the tonic inhibition exerted by the cytoplasmic cyclic nucleotide binding domain on the channel pore (5). HCN channels expressed in dorsal root ganglia neurons may play a role in acute inflammatory pain (6). Cardiac HCN channels ensure electrical rhythmicity in cardiomyocytes (7). Alternate Names BCNG-2; BCNG2; brain cyclic nucleotide gated channel 2; Brain cyclic nucleotide-gated channel 2; HAC-1; HCN2; hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2; hyperpolarization activated cyclic nucleotide gated potassium channel 2; hyperpolarization activated cyclic nucleotide-gated potassium channel 2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG