CST, 14959S, CGRP (D5R8F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CGRP. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, CGRP (D5R8F) Rabbit Monoclonal Antibody (CST #14959) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:200 - 1:400 Immunofluorescence (Immunocytochemistry): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 69734 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CGRP (D5R8F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CGRP protein. Rat cross-reactivity is determined by Immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val114 of human CGRP protein. Background Calcitonin gene-related peptide (CGRP) is a peptide of 37 amino acids that belongs to the calcitonin (CT) family of peptide hormones. The calcitonin gene ( ) encodes a number of tissue-specific peptides through alternative splicing of mRNA transcripts and precursor protein cleavage (1). Both calcitonin and α-CGRP are produced from the CALCA gene, while a second gene ( ) encodes the related β-CGRP protein (2). α-CGRP and β-CGRP share similar activities and differ by three or fewer residues depending on the species (3). The CGRP peptide activates a heterotrimeric receptor complex that consists of the seven transmembrane-spanning calcitonin receptor-like receptor, the single transmembrane-spanning RAMP1 protein, and an intracellular receptor component protein (4,5). CGRP is expressed in the central and peripheral nervous system in mammals, where it exhibits several important physiologic roles. Research studies demonstrate that CGRP is a potent vasodilatator (6) and a modulator of acetylcholine receptor function at neuromuscular junctions (7). Additional studies indicate that CGRP peptide is involved in feeding (8) and inflammatory pain (9). CGRP peptide also plays a key role in the physiology of migraine attacks. Specifically, CGRP peptide levels increase during acute migraine attacks, which can be ameliorated through treatment with CGRP antagonists (10). Alternate Names Alpha-type CGRP; CALC1; CALCA; CALCA iso3; calcitonin; calcitonin 1; Calcitonin gene-related peptide 1; Calcitonin gene-related peptide I; calcitonin related polypeptide alpha; calcitonin-related polypeptide alpha; calcitonin/calcitonin-related polypeptide, alpha; CGRP; CGRP-alpha; CGRP-I; CGRP1; CT; katacalcin; KC; MGC126648; PCT Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 5 Source/Isotype: Rabbit IgG