CST, 14963T, PROX1 (D2J6J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PROX1. Validated for WB,WB,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, PROX1 (D2J6J) Rabbit Monoclonal Antibody (CST #14963) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunohistochemistry (Paraffin): 1:1000 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:12800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PROX1 (D2J6J) Rabbit Monoclonal Antibody recognizes endogenous levels of total PROX1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu567 of human PROX1 protein. Background Prospero homeobox protein 1 (PROX1) is a transcription factor known for its roles in organ development and lymphangiogenesis. It plays a critical role in the development of the CNS, lens, retina, liver, pancreas, and heart, and is considered to be the master regulator of the lymphatic system (1,2). PROX1 initiates the differentiation of lymphatic vasculature from the cardinal vein, where it is regulated by Sox18 (3,4). The PROX1 suppressor COUP-TFII represses the Notch pathway in venous endothelium, which prevents arterialization (4). HIF-1α and HIF-1β mediated hypoxia induces PROX1, which suggests a means of promoting lymphangiogenesis. Since the tumor microenvironment is typically hypoxic, regulation of PROX1 by hypoxia may also explain the up-regulation of this transcription factor in some cancers (2). PROX1 promotes colon cancer progression by down-regulating E-cadherin via miR-9, which promotes epithelial-mesenchymal transition (EMT) and metastasis (5). The PROX1 protein can act as a tumor suppressor in cases of hepatocellular carcinoma. PROX1 represses transcription of TWIST1, a transcription factor that promotes metastasis by binding the E-cadherin promoter. The function of PROX1 in other cancers is an area of active research (6). Alternate Names Homeobox prospero-like protein PROX1; prospero homeobox 1; Prospero homeobox protein 1; prospero-related homeobox 1; PROX-1; PROX1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 85 Source/Isotype: Rabbit IgG