CST, 14976T, CDCA2 (D7T4P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CDCA2/Repo-Man. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CDCA2 (D7T4P) Rabbit Monoclonal Antibody (CST #14976) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CDCA2 (D7T4P) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDCA2 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr50 of human CDCA2 protein. Background Cell division cycle associated 2 (CDCA2, Repo-Man) is a cell-cycle protein that recruits protein phosphatase 1 (PP1) to mitotic chromatin at anaphase onset, which is essential for cell proliferation (1). Carboxy-terminal phosphorylation of CDCA2 at Ser893 by Aurora B inhibits the protein and leads to diffuse localization during prometaphase and metaphase. Dephosphorylation of CDCA2 by PP2A is necessary for CDCA2/PP1 complex reformation (2). The CDCA2/PP1 complex is required for chromatin binding and dephosphorylation of histone H3 at Thr3, Ser10, and Ser28 (2-4). The CDCA2/PP1 complex is also involved in nuclear envelope reformation during mitotic exit for proper progression through the M/G1 transition (4). The interaction of CDCA2 with importin beta and Nup153, which is required for nuclear envelope formation, is negatively regulated by CDK phosphorylation of the amino-terminal domain of CDCA2 (5). CDCA2 may play a role in DNA repair as the release of CDCA2 from chromatin at sites of DNA damage promotes the activation of DNA damage response (6). These results imply that the CDCA2/PP1 complex may play a part in cancer progression. Research studies indicate that CDCA2 may serve as a prognostic marker, as increased CDCA2 expression is seen in a number of cancers, including melanoma, neuroblastoma tumors, squamous cell carcinoma, and synovial sarcomas (7-9). Alternate Names CDCA2; cell division cycle associated 2; Cell division cycle-associated protein 2; FLJ25804; MGC129906; MGC129907; PPP1R81; protein phosphatase 1 [gamma] (PPP1R3A) binding protein Repo-Man; protein phosphatase 1, regulatory subunit 81; Recruits PP1 onto mitotic chromatin at anaphase protein; Repo-Man Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 140-150 Source/Isotype: Rabbit IgG