{"product_id":"cst-14976t","title":"CST,  14976T, CDCA2 (D7T4P) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CDCA2\/Repo-Man. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CDCA2 (D7T4P) Rabbit Monoclonal Antibody (CST #14976) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCDCA2 (D7T4P) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDCA2 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr50 of human CDCA2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCell division cycle associated 2 (CDCA2, Repo-Man) is a cell-cycle protein that recruits protein phosphatase 1 (PP1) to mitotic chromatin at anaphase onset, which is essential for cell proliferation (1). Carboxy-terminal phosphorylation of CDCA2 at Ser893 by Aurora B inhibits the protein and leads to diffuse localization during prometaphase and metaphase. Dephosphorylation of CDCA2 by PP2A is necessary for CDCA2\/PP1 complex reformation (2). The CDCA2\/PP1 complex is required for chromatin binding and dephosphorylation of histone H3 at Thr3, Ser10, and Ser28 (2-4). The CDCA2\/PP1 complex is also involved in nuclear envelope reformation during mitotic exit for proper progression through the M\/G1 transition (4). The interaction of CDCA2 with importin beta and Nup153, which is required for nuclear envelope formation, is negatively regulated by CDK phosphorylation of the amino-terminal domain of CDCA2 (5). CDCA2 may play a role in DNA repair as the release of CDCA2 from chromatin at sites of DNA damage promotes the activation of DNA damage response (6). These results imply that the CDCA2\/PP1 complex may play a part in cancer progression. Research studies indicate that CDCA2 may serve as a prognostic marker, as increased CDCA2 expression is seen in a number of cancers, including melanoma, neuroblastoma tumors, squamous cell carcinoma, and synovial sarcomas (7-9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCDCA2; cell division cycle associated 2; Cell division cycle-associated protein 2; FLJ25804; MGC129906; MGC129907; PPP1R81; protein phosphatase 1 [gamma] (PPP1R3A) binding protein Repo-Man; protein phosphatase 1, regulatory subunit 81; Recruits PP1 onto mitotic chromatin at anaphase protein; Repo-Man\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 140-150\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797620838569,"sku":"14976T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14976t","provider":"Iright","version":"1.0","type":"link"}