{"product_id":"cst-14977s","title":"CST,  14977S, CENP-E Antibody","description":"Polyclonal Antibody for studying CENPE. Validated for Western Blotting. Highly specific and rigorously validated in-house, CENP-E Antibody (CST #14977) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCENP-E Antibody recognizes endogenous levels of total CENP-E protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1897 of human CENP-E protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCentromere-associated protein E (CENP-E) is a kinesin-like motor protein and mitotic-checkpoint kinase BUB1B binding partner that is essential for establishing and maintaining stable attachments between mitotic chromosomes and spindle microtubules (1). CENP-E plays an important role as a motor protein in the alignment of chromosomes during prometaphase (2). Research studies indicate that CENP-E protein expression peaks in late G2 and M-phases of the cell cycle before the protein is degraded at mitotic exit (3). Additional studies show that the loss of CENP-E function results in cell cycle arrest in mitosis. Mutations in the corresponding gene can result in autosomal recessive primary microcephaly-13, a developmental disorder characterized by small head circumference, dysmorphic facial features, short stature, and delayed psychomotor development (4). Since CENP-E is essential for mitotic progression and is required for cellular proliferation, it has become an interesting target for cancer therapy (5-7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCENP-E; CENPE; Centromere autoantigen E (312kD); Centromere protein E; centromere protein E, 312kDa; Centromere-associated protein E; KIF10; kinesin family member 10; Kinesin-7; Kinesin-related protein CENPE; MCPH13; PPP1R61; protein phosphatase 1, regulatory subunit 61\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 270\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797620871337,"sku":"14977S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14977s","provider":"Iright","version":"1.0","type":"link"}