{"product_id":"cst-14989s","title":"CST,  14989S, PTMScan® Phospho-Ser-Pro-Pro Motif [pSPP] Kit","description":"PTMScan for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nCells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan ® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS\/MS analysis. CST recommends the use of PTMScan ® IAP Buffer #9993 included in the kit.\n\u003cb\u003eStorage\u003c\/b\u003e\nAntibody beads supplied in IAP buffer containing 50% glycerol. Store at -20ºC. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: PTMScan\n\u003cb\u003eBackground\u003c\/b\u003e\nThe MAPK and CDK families of serine\/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To facilitate the study and discovery of new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine or phospho-serine followed by proline. The S\/TPP motif matches the consensus sequence for targeted phosphorylation by Cdc2 (7,8), ERK1, ERK2 (9), SAPK\/JNK, CDK5, and GSK3 (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nSPP","brand":"CST","offers":[{"title":"Default Title","offer_id":46800793338025,"sku":"14989S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14989s","provider":"Iright","version":"1.0","type":"link"}