{"product_id":"cst-14999t","title":"CST,  14999T, MTHFD1L (D8T7L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MTHFD1L. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MTHFD1L (D8T7L) Rabbit Monoclonal Antibody (CST #14999) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMTHFD1L (D8T7L) Rabbit Monoclonal Antibody recognizes endogenous levels of total MTHFD1L protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala264 of human MTHFD1L protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNADP+ dependent methylenetetrahydrofolate dehydrogenase 1-like (MTHFD1L) is a mitochondrial enzyme that catalyzes the production of formate from 10-formyl-tetrahydrofolate, the last step in one-carbon (1-C) flow from mitochondria to cytoplasm (1,2). These one-carbon end products are required for synthesis of thymidylate and purines. In the mitochondria, these essential one-carbon products are formed by a series of reactions catalyzed by a pair of enzymes (MTHFD2 and MTHFD1L), but by the trifunctional MTHFD1 enzyme in the cytoplasm (3). The 10-formyl-tetrahydrofolate synthetase MTHFD1L is widely expressed in most adult tissues and at all stages of mammalian embryonic development (1). Research studies using MTHFD1L knockout mice indicate that MTHFD1L plays an essential role in neural tube formation; mice lacking MTHFD1L displayed neural tube and craniofacial defects leading to embryonic lethality (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n10-formyl-THF synthetase; C1TM; dJ292B18.2; DKFZp586G1517; FLJ21145; Formyltetrahydrofolate synthetase; formyltetrahydrofolate synthetase domain containing 1; FTHFSDC1; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like; mitochondrial C1-tetrahydrofolate synthase; mitochondrial C1-tetrahydrofolate synthetase; Monofunctional C1-tetrahydrofolate synthase, mitochondrial; MTC1THFS; MTHFD1L\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 106\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797621592233,"sku":"14999T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-14999t","provider":"Iright","version":"1.0","type":"link"}