CST, 15076S, DDX41 (D3F1Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DDX41. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, DDX41 (D3F1Z) Rabbit Monoclonal Antibody (CST #15076) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:200 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DDX41 (D3F1Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total DDX41 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala98 of human DDX41 protein. Background The DEAD box helicase DDX41 is an antiviral protein that senses cytosolic DNA and activates the signaling adaptor STING to induce type I interferon production (1). The recognition of bacterial dinucleotides by DDX41 also leads to STING-dependent type 1 interferon production (2). Research studies demonstrate that knockdown of DDX41 attenuates poly(dA:dT)-induced IFN-β production and cyclic GMP-AMP synthase (cGAS, MB21D1) induction (3). The DDX41 protein is thought to be an initial constitutively expressed DNA sensor that activates type I interferon production and leads to cGAS amplification of type I interferon production (3). DDX41 is negatively regulated by the E3 ligase TRIM21 (4). Both germline and somatic mutations in the corresponding gene are associated with myeloid neoplasms (5,6). Alternate Names 2900024F02Rik; ABS; DDX41; DEAD (Asp-Glu-Ala-Asp) box polypeptide 41; DEAD box protein 41; DEAD box protein abstrakt homolog; DEAD-box helicase 41; DEAD-box protein abstrakt; MGC8828; MPLPF; Probable ATP-dependent RNA helicase DDX41; putative RNA helicase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG