{"product_id":"cst-15101s","title":"CST,  15101S, NLRP3 (D4D8T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NLRP3 mouse. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, NLRP3 (D4D8T) Rabbit Monoclonal Antibody (CST #15101) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNLRP3 (D4D8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRP3 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala306 of mouse NLRP3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NACHT domain and carboxy-terminal leucine-rich repeats (LRR) involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7). NLRP3 is an inflammasome-forming NLR that activates caspase-1 leading to maturation of IL-1β and IL-18 (8). The NLRP3 inflammasome is assembled in response to a wide variety of microbial and endogenous stimuli, and therefore it is unlikely the NLRP3 directly interacts with its activators (8). NLRP3 is expressed at highest levels in dendritic cells, monocytes, and macrophages (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAGTAVPRL; AII\/AVP; Cias1; cold autoinflammatory syndrome 1 homolog; Cold autoinflammatory syndrome 1 protein homolog; cry; Cryopyrin; FCAS; FCU; mast cell maturation inducible protein 1; Mast cell maturation-associated-inducible protein 1; MGC129375; Mmig; Mmig1; MWS; NACHT, LRR and PYD domains-containing protein 3; NACHT\/LRR\/pyrin domain-containing protein 3; Nalp3; NLR family, pyrin domain containing 3; Nlrp3; OTTMUSP00000005970; Pyp; Pypaf1; PYRIN-containing APAF1-like protein 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 110\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797623066793,"sku":"15101S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-15101s","provider":"Iright","version":"1.0","type":"link"}