CST, 15107T, TREX1 (D8E2O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TREX1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TREX1 (D8E2O) Rabbit Monoclonal Antibody (CST #15107) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TREX1 (D8E2O) Rabbit Monoclonal Antibody recognizes endogenous levels of total TREX1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr241 of human TREX1 protein. Background TREX1 is a broadly expressed 3' to 5' exonuclease that acts on single-stranded DNA (ssDNA) to negatively regulate the interferon-stimulatory DNA (ISD) response (1-4). Three human TREX1 isoforms are generated through alternative splicing and have predicted molecular weights of 32 kDa, 33 kDa, and 39 kDa (2). The transcript for the 33 kDa isoform is the most abundant (2). TREX1-deficient mice accumulate intracellular ssDNA, which triggers the ISD response, ultimately resulting in lethal autoimmunity (3,4). mutations are associated with autoimmune diseases, including Aicardi-Goutieres syndrome and systemic lupus erythematosus (5,6). Additionally, TREX1 prevents the cell-intrinsic innate immune response to human immunodeficiency virus (HIV) by degrading excess HIV DNA that would otherwise trigger a type I interferon induction (7). Alternate Names 3' repair exonuclease 1; 3'-5' exonuclease TREX1; AGS1; CRV; Deoxyribonuclease III; DNase III; DRN3; HERNS; Three prime repair exonuclease 1; Three-prime repair exonuclease 1; TREX1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG