CST, 15108S, Smac/Diablo (D5S3R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Diablo/Smac. Validated for WB,IP,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Smac/Diablo (D5S3R) Rabbit Monoclonal Antibody (CST #15108) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:500 - 1:2000 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Smac/Diablo (D5S3R) Rabbit Monoclonal Antibody recognizes endogenous levels of total Smac/Diablo protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Smac/Diablo protein. Background Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6). Alternate Names 0610041G12Rik; DBLOH; DFNA64; DIABLO; diablo homolog (Drosophila); Diablo homolog, mitochondrial; Diablo IAP-binding mitochondrial protein; diablo-like protein; DIABLO-S; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMAC3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Rabbit IgG