CST, 15140T, TRXR1 (D1T3D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRXR1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TRXR1 (D1T3D) Rabbit Monoclonal Antibody (CST #15140) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 94415 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity TRXR1 (D1T3D) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRXR1 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly412 of human TRXR1 protein. Background TRXR1 (thioredoxin reductase 1) is a selenocysteine-containing protein that is involved in redox homeostasis (1-6). Its canonical target is thioredoxin, another redox protein (1). Together, they are involved in many functions such as antioxidant regulation (3-6), cell proliferation (2,3,5), DNA replication (2,3), and transcription (3,5). TRXR1 is also capable of reducing a wide array of cellular proteins (1,3). Selenium deficiency, either by diet modification (2,6) or introduction of methylmercury (4), hinders proper expression and function of TRXR1. It is possible that this effect, which results in a higher oxidative state, is a result of the selenocysteine codon (UGA) being read as a STOP codon in the absence of adequate selenium (4). The functions of TRXR1 in cell proliferation and antioxidant defense make it a potential therapeutic target. Alternate Names Gene associated with retinoic and IFN-induced mortality 12 protein; Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; GRIM12; KDRF; KM-102-derived reductase-like factor; MGC9145; oxidoreductase; selenoprotein TXNRD1; testis tissue sperm-binding protein Li 46a; thioredoxin reductase 1; Thioredoxin reductase 1, cytoplasmic; thioredoxin reductase GRIM-12; Thioredoxin reductase TR1; TR; TR1; TRXR1; TXNR; TXNRD1 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG