CST, 15171S, FAM3C (D1S2D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FAM3C. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, FAM3C (D1S2D) Rabbit Monoclonal Antibody (CST #15171) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:300 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 83157 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity FAM3C (D1S2D) Rabbit Monoclonal Antibody recognizes endogenous levels of total FAM3C protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with other FAM3 family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly169 of human FAM3C protein. Background FAM3C, also known as ILEI (interleukin-like epithelial-to-mesenchymal transition [EMT] inducer), is a cytokine-like protein and member of the FAM3 family. FAM3C plays an important role in EMT and metastasis during cancer progression in human and mouse cells, and is highly expressed in human cancer (1,2). In colorectal cancer, researchers have indicated that FAM3C is a marker for EMT and tumor progression, and that high expression of FAM3C is predictive of poor prognosis (3). While EMT induction by FAM3C can be independent of TGF-beta, research studies have also shown TGF-beta-dependent regulation of FAM3C expression at the translational level in mouse and human cells (4,5). FAM3C has also been linked to regulation of osteoblast differentiation (6), and to accumulation of amyloid beta plaques in Alzheimer's disease (7). FAM3C exists in monomeric and in homodimeric form, and research shows that FAM3C homodimers contain its EMT-inducing and tumor promoting activity (8). Alternate Names FAM3 metabolism regulating signaling molecule C; FAM3C; family with sequence similarity 3 member C; family with sequence similarity 3, member C; GS3786; ILEI; interleukin-like EMT inducer; interleukin-like epithelial-mesenchymal transition inducer; predicted osteoblast protein; Protein FAM3C Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG