{"product_id":"cst-15230t","title":"CST,  15230T, HSD3B1 (F7Q2L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HSD3B1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HSD3B1 (F7Q2L) Rabbit Monoclonal Antibody (CST #15230) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHSD3B1 (F7Q2L) Rabbit Monoclonal Antibody recognizes endogenous levels of total HSD3B1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro154 of human HSD3B1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\n3 beta-hydroxysteroid dehydrogenase\/Delta 5--\u0026gt;4-isomerase type 1 (HSD3B1), also known as 3β-hydroxysteroid dehydrogenase-1 (3βHSD1), is critical for steroid hormone biosynthesis (1,2). This enzyme catalyzes the rate-limiting step of extragonadal androgen synthesis. Studies show that glucosamine released from cancer-associated fibroblasts (CAFs) enhances O-GlcNAcylation in prostate cancer cells, activating HSD3B1\/3βHSD1 expression mediated by the transcription factor Elk1. This increases intratumoral androgen biosynthesis and leads to castration-resistant prostate cancer (CRPC) (2). In addition, the adrenal-permissive (1245C) allele, which upregulates intratumoral androgen biosynthesis, augments resistance to radiotherapy in prostate cancer (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n3 beta-hydroxysteroid dehydrogenase\/Delta 5--\u0026gt;4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase\/Delta 5--\u0026gt;4-isomerase type I; 3-beta-HSD I; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxysteroid 3-dehydrogenase; 3BETAHSD; 3BH; 3BHS1; Delta-5-3-ketosteroid isomerase; Dihydrotestosterone oxidoreductase; HSD3B; HSD3B1; HSDB3; HSDB3A; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1; hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1; I; Progesterone reductase; SDR11E1; short chain dehydrogenase\/reductase family 11E, member 1; Steroid Delta-isomerase; Trophoblast antigen FDO161G\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 42\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797625163945,"sku":"15230T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-15230t","provider":"Iright","version":"1.0","type":"link"}