CST, 15269SF, Jagged1 (D4Y1R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Jagged1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Jagged1 (D4Y1R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #15269) is ready to ship. Product Usage Information This product is the carrier free version of product #70109. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 70109 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Jagged1 (D4Y1R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Jagged1 protein. Based on sequence analyses, this antibody is not predicted to detect Jagged2 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1131 of human Jagged1 protein. Background Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3, and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus, where it activates transcription. Notch ligands may also be processed in a way similar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). Alternate Names AGS; AGS1; AHD; AWS; CD339; DCHE; hJ1; JAG1; jagged 1 (Alagille syndrome); jagged canonical Notch ligand 1; Jagged1; JAGL1; MGC104644; Protein jagged-1 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 180 Source/Isotype: Rabbit IgG