CST, 15365S, DCP1A (D6V1R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DCP1A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, DCP1A (D6V1R) Rabbit Monoclonal Antibody (CST #15365) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DCP1A (D6V1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total DCP1A protein. This antibody may also detect a nonspecific band of about 85 kDa. This antibody does not cross-react with DCP1B protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu436 of human DCP1A protein. Background mRNA decapping is an important process in the mRNA turnover (1). DCP1A and DCP2 were identified as two human decapping enzymes and homologs of the better-characterized enzymes. Both putative decapping enzymes interact with the regulator of nonsense transcripts 1 (UPF1) and may be recruited by UPF1 or related proteins to mRNA sequences that contain premature termination codons (1). Additional research studies demonstrate that DCP1A, DCP1B (the homolog of DCP1A) and DCP2 colocalize with decapping activation factors RCK/p54 and Lsm proteins in cytoplasmic loci (2). DCP1A, DCP1B and DCP2 are components of cytoplasmic processing (P) bodies, with hyper-phosphorylation of DCP1A during mitosis suggesting a possible mechanism of P-body regulation during the cell cycle (3,4). Alternate Names DCP1 decapping enzyme homolog A; DCP1 decapping enzyme homolog A (S. cerevisiae); DCP1 decapping enzyme-like protein A; DCP1A; decapping enzyme hDcp1a; decapping mRNA 1A; FLJ21691; HSA275986; mRNA-decapping enzyme 1A; Nbla00360; putative protein product of Nbla00360; Smad4-interacting transcriptional co-activator; SMAD4IP1; SMIF; Transcription factor SMIF Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 73,75 Source/Isotype: Rabbit IgG