CST, 15413S, F3/Contactin (D7D4X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CNTN1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, F3/Contactin (D7D4X) Rabbit Monoclonal Antibody (CST #15413) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity F3/Contactin (D7D4X) Rabbit Monoclonal Antibody recognizes endogenous levels of total F3/contactin protein. This antibody detects a 32 kDa protein of unknown identity in some tissue extracts. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met774 of human F3/contactin protein. Background F3/contactin (CNTN, contactin 1) is a glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion protein belonging to the immunglobulin protein superfamily (1). During early mammalian development, F3/contactin is expressed in granule neuronal progenitor (GNP) cells, where it was shown to promote GNP differentiation, in part by antagonizing sonic hedgehog (SHH)-mediated proliferation (2). Biochemical studies have shown that F3/contactin interacts with the phosphatase PTPRZ on the surface of oligodendrocyte precursor cells, an association that was shown to be essential for oligodendrocyte maturation (3). F3/contactin expression is also abundant in post-mitotic neurons, where its functions as a neural cell adhesion protein have been suggested to play an important role in synaptic plasticity and memory (4). Although primarily associated with neuronal development and function, F3/contactin expression has also been implicated in extra-neuronal tumorigenesis. For example, expression of F3/contactin was detected in both primary prostate tumors, and lymph node and bone metastases, while patient tumor samples with detectable F3/contactin expression were associated with tumor progression and reduced recurrance-free survival (5). Alternate Names CNTN1; contactin 1; Contactin-1; F3; Glycoprotein gp135; GP135; MYPCN; Neural cell surface protein F3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG