{"product_id":"cst-15446t","title":"CST,  15446T, Phospho-Jak2 (Ser523) (F8E8U) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Jak2 (Ser523) phosphate. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Jak2 (Ser523) (F8E8U) Rabbit Monoclonal Antibody (CST #15446) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Jak2 (Ser523) (F8E8U) Rabbit Monoclonal Antibody recognizes endogenous levels of Jak2 protein only when phosphorylated at Ser523.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser523 of human Jak2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMembers of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. The JH2 domain of Jak2 has been shown to autophosphorylate two negative regulatory sites on Jak2, Ser523 and Tyr570. In the absence of cytokine stimulation, Jak2 is constitutively phosphorylated on Ser523, effectively inhibiting its activity (6,7). Mutation in the JH2 domain can lead to an increase in Jak2 activity, resulting in myeloproliferative neoplasms (MPNs) (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nJAK-2; JAK2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK10; Tyrosine-protein kinase JAK2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 125\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797626376361,"sku":"15446T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-15446t","provider":"Iright","version":"1.0","type":"link"}