CST, 15503SF, TRBC1/TCR beta constant region 1 (E6Z3S) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TCRbeta. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, TRBC1/TCR beta constant region 1 (E6Z3S) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #15503) is ready to ship. Product Usage Information This product is the carrier free version of product #79485. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 79485 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TRBC1/TCR beta constant region 1 (E6Z3S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TRBC1/TCRβ constant region 1 protein. This antibody does not cross-react with TRBC2/TCRβ constant region 2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TRBC1/TCRβ constant region 1 protein. Background The αβ T Cell Receptor (TCR) is a heterodimer composed of an alpha chain and a beta chain expressed on the surface of T cells (1,2). It detects peptide antigen presented by major histocompatibility complex (MHC) molecules (1,2). Detection of cognate antigen leads to activation of the TCR signaling pathway through the CD3 signaling chains that associate with the TCR (1). The TCR chains are made up of a constant region and variable regions that get rearranged during T cell development in the thymus (3). There are two TCRβ constant genes, and (3). A population of normal T cells is polyclonal and contains a mix of TRBC1- and TRBC2-expressing T cells, while T cell malignancies are monoclonal and all express the same TCR which either contains TRBC1 or TRBC2 (4). Therefore, it is possible that T cell malignancies could be identified and targeted with reagents or therapies that can distinguish between TRBC1 and TRBC2 (4). Alternate Names BV05S1J2.2; MGC88817; T cell receptor beta constant 1; T-cell receptor beta-1 chain C region; TCR-beta1; TCRB; TCRBC1; TCRbeta; TCRbeta1; TRBC1; V_segment translation product Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30-40 Source/Isotype: Rabbit IgG