{"product_id":"cst-15585s","title":"CST,  15585S, CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PECAM-1 mouse. Validated for WB,WB,IF,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody (CST #15585) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunofluorescence (Frozen): 1:200 - 1:400\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:50 - 1:200\nFlow Cytometry (Live): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD31 (PECAM-1) protein.\nSpecies Reactivity: Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse CD31 (PECAM-1) protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC85791; Cd31; MGC102160; OTTMUSP00000003452; OTTMUSP00000003454; PECA1; Pecam; PECAM-1; PECAM-1\/CD31; Pecam1; Platelet endothelial cell adhesion molecule; platelet\/endothelial cell adhesion molecule 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M R\nSENSITIVITY: Endogenous\nMW (kDa): 135\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801048993961,"sku":"15585S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-15585s","provider":"Iright","version":"1.0","type":"link"}