CST, 15616T, SPT6 (D6J9H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SPT6. Validated for WB,IP,ChIP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, SPT6 (D6J9H) Rabbit Monoclonal Antibody (CST #15616) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:200 Chromatin IP: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP, CUT&RUN, CUT&Tag Specificity / Sensitivity SPT6 (D6J9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPT6 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val112 of human SPT6 protein. Background SPT6 or SUPT6H is a histone H3 chaperone protein involved in transcriptional elongation and chromatin structure (1). The SPT6 protein contains a highly acidic N-terminus with leucine zipper and SH2 domains, which can interact with phospho-Rpb1 CTD (Ser2) to recruit SPN1 and other mRNA processing and export factors (2). SPT6 can enhance the elongation rate of RNA polymerase II, and can also maintain the modification state of histone H3 tails. (3-4). Loss of SPT6 causes improper initiation of transcription within coding regions (5). Alternate Names emb-5; Histone chaperone suppressor of Ty6; hSPT6; KIAA0162; MGC87943; SPT6; SPT6 homolog, histone chaperone and transcription elongation factor; SPT6H; suppressor of Ty 6 homolog; suppressor of Ty 6 homolog (S. cerevisiae); SUPT6H; Tat-cotransactivator 2 protein; Tat-CT2 protein; Transcription elongation factor SPT6 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 210 Source/Isotype: Rabbit IgG