CST, 15742S, HNF-4 gamma (E4V2B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HNF4G. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, HNF-4 gamma (E4V2B) Rabbit Monoclonal Antibody (CST #15742) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity HNF-4 gamma (E4V2B) Rabbit Monoclonal Antibody recognizes endogenous levels of total HNF4γ protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val363 of human HNF4γ protein. Background Hepatocyte nuclear factor 4γ (HNF4γ) is a transcription factor that belongs to the orphan nuclear receptor superfamily (1). Studies of knockout mice show that HNF4γ is involved in the regulation of glucose homeostasis (2,3). In addition, HNF4γ expression was shown to be upregulated in lung cancer. Overexpressed HNF4γ stimulates lung cancer cell proliferation (1). Furthermore, an aberrant gastrointestinal-lineage transcriptome was identified in about 30% of castration-resistant prostate cancers. HNF4γ, along with HNF1α, regulates this transcriptome. HNF4γ generates and maintains enhancers at the gastrointestinal-lineage genes as a pioneer factor and mediates castration resistance (4). Alternate Names hepatocyte nuclear factor 4 gamma; Hepatocyte nuclear factor 4-gamma; hepatocyte nuclear factor 4, gamma; HNF-4-gamma; HNF4G; NR2A2; NR2A3; Nuclear receptor subfamily 2 group A member 2 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 46 Source/Isotype: Rabbit IgG