CST, 15793S, MTA2 Antibody

Polyclonal Antibody for studying MTA2. Validated for Western Blotting. Highly specific and rigorously validated in-house, MTA2 Antibody (CST #15793) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MTA2 Antibody recognizes endogenous levels of total MTA2 protein. This antibody does not cross-react with other MTA proteins. This antibody detects a 50 kDa band of unknown origin in rat cell lysates. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro602 of human MTA2 protein. Antibodies are purified by peptide affinity chromatography. Background MTA2 is a component of the NuRD deacetylase complex, which is recruited by MBD3 and modulates the enzymatic activity of the histone deacetylase core (1,2). MTA2 is highly related to MTA1, but both proteins exist in biologically distinct complexes, with MTA2 being associated with complexes containing Sin3a and CHD4 (3) MTA2 has been shown to bind ERα and repress its activity and contributes to estradiol- and tamoxifen-resistant breast cancers (4). Indeed, the MTA proteins are critical regulators of many biological functions in both cancer and non-cancer settings (5,6). MTA2 upregulation has been reported in a multitude of cancers, including hepatocellular carcinomas, colorectal cancers, gastric cancers, and esophageal cancers (7-10). MTA2 has also been described in B cell development, where it interacts with Aiolos and Ikaros to control target genes (11). Alternate Names DKFZp686F2281; metastasis -associated gene 1-like 1; metastasis associated 1 family member 2; metastasis associated 1 family, member 2; metastasis associated gene family, member 2; Metastasis-associated 1-like 1; metastasis-associated protein 2; Metastasis-associated protein MTA2; MTA1-L1 protein; MTA1L1; MTA2; p53 target protein in deacetylase complex; PID Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 75 SOURCE: Rabbit