CST, 15800S, uPA (E2M6I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying uPA. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, uPA (E2M6I) Rabbit Monoclonal Antibody (CST #15800) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity uPA (E2M6I) Rabbit Monoclonal Antibody recognizes endogenous levels of total uPA protein. This antibody detects the 50 kDa zymogen and the 32 kDa chain B, and does not detect chain A. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser266 of human uPA protein. Background uPA (PLAU, urokinase-type plasminogen activator) is a secreted serine protease that plays an important role in plasminogen activation (1). uPA exists as a secreted 50 kDa inactive zymogen. Upon binding to its high affinity serine protease urokinase-type plasminogen activator receptor (uPAR), it is processed to two interlinked chains, 18 kDa chain A and 32 kDa chain B, and becomes activated to convert plasminogen to its active form, plasmin, in a spatially restricted manner on the cell surface (2). Plasmin then carries out its protease activity toward a wide range of substrates. uPA activity is inhibited by serpins (serine protease inhibitors), such as plasminogen activator inhibitor (PAI) (3). uPA, uPAR, and PAI form a Urokinase Plasminogen Activation System (uPAS) (4). This system plays a key role in regulating a broad range of biological processes under normal physiological conditions, such as tissue wound healing (5), cell migration (6), fibrinolysis (7), and angiogenesis (8). The system also contributes to disease progression, such as cancer development (9), neurodegeneration disorders (10), inflammatory lung disease, and rheumatoid arthritis (11,12). Alternate Names ATF; BDPLT5; plasminogen activator, urinary; plasminogen activator, urokinase; PLAU; QPD; u-PA; U-plasminogen activator; uPA; URK; UROK; Urokinase-type plasminogen activator; Urokinase-type plasminogen activator chain B; Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 32, 50 Source/Isotype: Rabbit IgG