CST, 15969S, ZBTB24 (E5D6G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZBTB24. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, ZBTB24 (E5D6G) Rabbit Monoclonal Antibody (CST #15969) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity ZBTB24 (E5D6G) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZBTB24 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro569 of human ZBTB24 protein. Background Zinc finger and BTB domain-containing protein 24 (ZBTB24), also called ZNF450, is a DNA-binding protein initially found to be expressed in response to BMP-2 signaling (1). ZBTB24 along with CDCA7 play a role in DNA methylation by recruiting the HELLS chromatin remodeling complex to centromeric satellite repeats (2). Mutations in are linked to immunodeficiency and centromeric instability, and facial anomalies syndrome type 2 (ICF2) (3,4). ZBTB24 is recruited to DNA breaks by PARP1 and promotes assembly of the LIG4/XRCC4 complex, resulting in error free non-homologous end joining (NHEJ) (5). ZBTB24 has also been shown to play a role in trophoblast proliferation through the methylation of E-cadherin (6) Alternate Names Bax-interacting factor-1; BIF1; ICF2; KIAA0441; PATZ2; POZ (BTB) and AT hook containing zinc finger 2; ZBT24; ZBTB24; zinc finger and BTB domain containing 24; Zinc finger and BTB domain-containing protein 24; Zinc finger protein 450; ZNF450 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG