{"product_id":"cst-15969s","title":"CST,  15969S, ZBTB24 (E5D6G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ZBTB24. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, ZBTB24 (E5D6G) Rabbit Monoclonal Antibody (CST #15969) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nChromatin IP: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nZBTB24 (E5D6G) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZBTB24 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro569 of human ZBTB24 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nZinc finger and BTB domain-containing protein 24 (ZBTB24), also called ZNF450, is a DNA-binding protein initially found to be expressed in response to BMP-2 signaling (1). ZBTB24 along with CDCA7 play a role in DNA methylation by recruiting the HELLS chromatin remodeling complex to centromeric satellite repeats (2). Mutations in are linked to immunodeficiency and centromeric instability, and facial anomalies syndrome type 2 (ICF2) (3,4). ZBTB24 is recruited to DNA breaks by PARP1 and promotes assembly of the LIG4\/XRCC4 complex, resulting in error free non-homologous end joining (NHEJ) (5). ZBTB24 has also been shown to play a role in trophoblast proliferation through the methylation of E-cadherin (6)\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBax-interacting factor-1; BIF1; ICF2; KIAA0441; PATZ2; POZ (BTB) and AT hook containing zinc finger 2; ZBT24; ZBTB24; zinc finger and BTB domain containing 24; Zinc finger and BTB domain-containing protein 24; Zinc finger protein 450; ZNF450\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 95\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797628375209,"sku":"15969S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-15969s","provider":"Iright","version":"1.0","type":"link"}