{"product_id":"cst-16016w2","title":"CST,  16016W2, Phospho-ATF-2 (Thr71)\/ATF-7 (Thr53) (A8J7P) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 532 Conjugate)","description":"Monoclonal Antibody for studying ATF7 (Thr53) phosphate\/ATF-2 (Thr71) phosphate. Highly specific and rigorously validated in-house, Phospho-ATF-2 (Thr71)\/ATF-7 (Thr53) (A8J7P) Rabbit Monoclonal Antibody (SignalFlex\u003csup\u003e™\u003c\/sup\u003e Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 532 Conjugate) (CST #16016) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions\/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-ATF-2 (Thr71)\/ATF-7 (Thr53) (A8J7P) Rabbit mAb (SignalFlex™ Alexa Fluor\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF-2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF\/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK\/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2). ATF-7 is another member of the ATF\/CREB family of leucine zipper proteins (5). Similarly, Thr51 and Thr53 (corresponding to Thr69 and Thr71 of ATF-2, respectively) can be phosphorylated under different conditions (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nActivating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; Activating transcription factor 7; ATF-2; ATF2; ATF7; ATFA; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-7; cAMP-responsive element-binding protein 2; CRE-BP1; CREB-2; CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-dependent transcription factor ATF-7; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; MGC57182; Transcription factor ATF-A; TREB7\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800350085289,"sku":"16016W2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-16016w2","provider":"Iright","version":"1.0","type":"link"}